Which of the following is obtained using processed mRNA molecules as a template

Using processed mRNA molecules as a template, a cDNA (complementary DNA) library can be obtained. This is a collection of DNA sequences that represent the mRNA molecules expressed in a given cell, tissue, or organism at a specific time. Here's how cDNA is made from mRNA and what it is used for:

1. Isolation of mRNA: Processed mRNA molecules are isolated from the cells or tissues of interest. These mRNA molecules contain only the coding regions (exons) of genes, as introns and other non-coding sequences have been removed during RNA processing.

2. Reverse Transcription: The mRNA molecules are then used as templates for the synthesis of complementary DNA (cDNA) using an enzyme called reverse transcriptase. This enzyme synthesizes a DNA strand that is complementary to the mRNA sequence.

3. Second Strand Synthesis: Once the first strand of cDNA is synthesized, a second strand of DNA can be synthesized to create double-stranded cDNA.

4. Cloning: The cDNA can then be cloned into a vector such as a plasmid or bacteriophage for storage, propagation, and further analysis.

5. Applications: A cDNA library represents the expressed genes in the original cells or tissues and is used in a variety of applications, including gene expression studies, functional genomics, and the identification of novel genes or gene variants.

In summary, cDNA is obtained using processed mRNA molecules as a template through reverse transcription and is a valuable tool in molecular biology and genomics research.